Probing CpG Methylation at CACGTG with BbrPI restriction enzyme
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منابع مشابه
Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods
Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods Michael Stevens, Jeffrey B. Cheng, Daofeng Li, Mingchao Xie, Chibo Hong, Cécile L. Maire, Keith L. Ligon, Martin Hirst, Marco A. Marra, Joseph F. Costello, and Ting Wang Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University...
متن کاملMethylCRF, an Algorithm for Estimating Absolute Methylation Levels at Single CpG Resolution from Methylation Enrichment and Restriction Enzyme Sequencing Methods
Recent advancements in sequencing-based DNA methylation profiling methods provide an unprecedented opportunity to map complete DNA methylomes. These include whole-genome bisulfite sequencing (WGBS, MethylC-seq, or BS-seq), reduced-representation bisulfite sequencing (RRBS), and enrichment-based methods such as MeDIP-seq, MBD-seq, and MRE-seq. These methods yield largely comparable results but d...
متن کاملTitle: Estimating Absolute Methylation Levels at Single CpG Resolution from Methylation Enrichment and Restriction Enzyme Sequencing Methods Running title: methylCRF predicts single CpG resolution methylome
1 Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63108 2 Department of Computer Science and Engineering, Washington University in St. Louis, St. Louis, MO 63130 3 Department of Dermatology, University of California San Francisco, CA 94143 4 Brain Tumor Research Center, Department of Neurosurgery, Helen Diller Famil...
متن کاملThe restriction enzyme EheI (GGC/GCC) is sensitive to CpG methylation.
Ehel cleaves the site GGC/GCC and is an isoschizomer of Narl (GG/CGCQ and Bbel (GGCGC/C) (1 -4) . Here I show that Ehel is methyl sensitive and will not cleave its CpG methylated site (GC^CGCC). Narl and Bbel are also CpG methyl sensitive (5—7). Ehel methyl sensitivity is shown by digesting mixtures of methylated X DNA and unmethylated plasmid DNA and viceversa (Figure 1). I also digest eukaryo...
متن کاملIn Silico Enhanced Restriction Enzyme Based Methylation Analysis of the Human Glioblastoma Genome Using Agilent 244K CpG Island Microarrays
Genome wide methylation profiling of gliomas is likely to provide important clues to improving treatment outcomes. Restriction enzyme based approaches have been widely utilized for methylation profiling of cancer genomes and will continue to have importance in combination with higher density microarrays. With the availability of the human genome sequence and microarray probe sequences, these ap...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.12.2950